Development and characterization of polymorphic microsatellite markers in taro (Colocasia esculenta).

نویسندگان

  • Emma S Mace
  • Ian D Godwin
چکیده

Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection.

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منابع مشابه

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عنوان ژورنال:
  • Genome

دوره 45 5  شماره 

صفحات  -

تاریخ انتشار 2002